植物性神经对围着床期大鼠子宫中肥大细胞和组织胺含量的影响

doi:10.11843/j.issn.0366-6964.2018.11.012

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (11): 2409-2415.doi: 10.11843/j.issn.0366-6964.2018.11.012

植物性神经对围着床期大鼠子宫中肥大细胞和组织胺含量的影响

黄丽波¹, 张秀霞², 范晓杰², 侯衍猛¹, 姜淑贞¹, 鲁璇¹, 袁学军^3*

1. 山东农业大学动物科技学院, 山东省动物生物工程与疾病防治重点实验室, 山东省畜禽疫病防制工程技术研究中心, 泰安 271018;
2. 山东省农业科学院, 济南 250014;
3. 山东农业大学生命科学学院, 泰安 271018

收稿日期:2018-01-30 出版日期:2018-11-23 发布日期:2018-11-23
通讯作者: 袁学军,副教授,硕士生导师,E-mail:xjyuan@sdau.edu.cn
作者简介:黄丽波(1969-),女,黑龙江依安人,博士,副教授,硕士生导师,主要从事动物生殖与免疫研究,E-mail:huanglibo227@126.com
基金资助:
山东省自然科学基金资助项目（ZR2010CM052）；山东省教育厅高等学校科技计划资助项目（J11LC26）

Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation

HUANG Li-bo¹, ZHANG Xiu-xia², FAN Xiao-jie², HOU Yan-meng¹, JIANG Shu-zhen¹, LU Xuan¹, YUAN Xue-jun^3*

1. Shandong Livestock and Poultry Disease Prevention and Control Engineering Technology Research Center, Shandong Key Laboratory of Animal Bioengineering and Disease Prevention and Control, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Academy of Agricultural Sciences, Ji'nan 250014, China;
3. College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China

Received:2018-01-30 Online:2018-11-23 Published:2018-11-23

摘要/Abstract

摘要：

本研究旨在以肥大细胞和组织胺为指标探讨大鼠围着床期植物性神经与子宫局部细胞免疫的关系。选择180日龄，体重为300～350 g的性成熟Wistar雌性大鼠90只，随机分为对照、假手术处理和手术处理（n=30）3组，手术处理为孕前切除支配子宫的植物性神经（肠系膜后神经节节后纤维和盆神经丛节前纤维），伤口愈合，开始合笼试验。在妊娠4～6 d，无菌条件下，取出大鼠子宫和卵巢，观察排卵、胚胎着床情况变化，通过细胞染色和ELISA方法测定肥大细胞数量和组织胺释放量。结果表明：正常大鼠子宫中组织胺自发释放量和总含量在着床（d5）时最低。肥大细胞数量也呈现相似的变化。神经切除后，自发释放组织胺量在妊娠4～6 d数值明显下降，且在妊娠4、6 d与对照组相比较差异显著（P<0.05）；可释放组织胺总含量在着床前（d4）极显著降低（P<0.01），且胚胎着床数量明显减少或延迟；肥大细胞数量在着床前后与组织胺总含量有相似的变化。这些结果说明，切除神经降低了着床前肥大细胞数量和组织胺水平，改变了子宫局部免疫水平，进而引起了胚胎着床减少或延迟。综上表明，支配子宫的植物性神经可在着床前上调子宫肥大细胞数量和组织胺水平，控制子宫局部免疫水平，在胚胎着床中起重要调控作用。

Abstract:

The objective of this study was to explore the relationship between autonomic nerve and cellular immune in uterus during peri-implantation based on mast cell (MC) count and histamine content. Ninety healthy female Wistar rats weighed 300-350 g were randomly assigned into 3 groups. One group as neurectomy (the autonomic nerves governing the uterus from the pelvis nerve and mesenteric caudal ganglion were amputated by surgical operation before pregnancy) experiment group, one group as sham operation group and the other as the control. After fully recovering from the surgery, female rat was cohabited with male rat in ovulatory period. On day 4, 5, 6 of pregnancy(d4, d5, d6), the females were killed by neck dislocation, uteri and ovary were surgically removed in abacterial condition. The number of ovulation sites was observed under an anatomy microscope and the quantity of embryos was counted, MC count was determined by cell staining, and histamine was assayed using histamine ELISA kits. The results showed that the spontaneous release and content of histamine in the uterus of normal rats were the lowest level at the time of implantation (d5). The MC count presented the similar changes to histamine. Compared with the control, spontaneous release of histamine from uterus in operated rat showed a significant decrease at d4, d6 of pregnancy (P<0.05), amputation of the nerve innerving uterus very significantly decreased the total content of histamine (P<0.01) before embryo implantation (d4), meanwhile the number of implanted embryo was also markedly decreased or delay. There were similar changes in the number of uterine MC and histamine content during implantation after neurectomy. These results suggested that resection of the nerve innerving uterus decreased MC counts and histamine level in the period of pre-implantation, changed uterine local immunity level, leading to reduction or delay of implantation. Taken together, our results imply that the autonomic nerve innerving uterus can up-regulate the local immune level in the the uterus through the proliferation of MC and histamine releasing, and play an important regulating role in embryo implantation.

中图分类号:

Q425

黄丽波, 张秀霞, 范晓杰, 侯衍猛, 姜淑贞, 鲁璇, 袁学军. 植物性神经对围着床期大鼠子宫中肥大细胞和组织胺含量的影响[J]. 畜牧兽医学报, 2018, 49(11): 2409-2415.

HUANG Li-bo, ZHANG Xiu-xia, FAN Xiao-jie, HOU Yan-meng, JIANG Shu-zhen, LU Xuan, YUAN Xue-jun. Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2409-2415.

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植物性神经对围着床期大鼠子宫中肥大细胞和组织胺含量的影响

Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation

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